Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Morphologic profiling of Sulfasalazine, DHA and the novel hybrid compound AC254 on rodent glioma cells. ( A ) Rat glioma cells (F98) were treated with a range of concentrations of SAS, DHA, AC254, SAS and solvent DMSO combined with DHA. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µM. ( B ) F98 were treated with 0.5 µM of SAS, DHA, AC254 and SAS combined with. Cells were fixated and stained with actin marker Phalloidin 488 and DNA marker Hoechst after 24 h of treatment. Axio Observer with Zen Software (Zeiss). Scale bar: 20 µm.

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software, Staining, Marker

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Effects on human cell viability (U87, TN22). ( A ) Cells (U87) were treated with 1 µM of the compounds. Cell viability was measured after 72 h. n = 4. * indicates significance to control ( p < 0.05). ( B ) U87 were treated with various concentrations of the compounds. Cell viability was measured the same way as in A. n = 4. * indicates significance to control ( p < 0.05). ( C ) TN22 were treated with 5 µM of the compounds. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µm. ( D ) TN22 were treated with 5 µM of the compounds, cell viability was measured after 72 h. n = 4. * indicates significance to control ( p < 0.05).

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Impact on human cell morphology. ( A ) Human cells (U87) were treated with a variation of concentrations of SAS, DHA, their covalently bound hybrid (AC254) and their 1:1 mixture. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µM. ( B ) Cells (U87) were treated with 5 µM of SAS, DHA, AC254 and SAS combined with. Cells were fixated and stained with actin marker Phalloidin 488 and DNA marker Hoechst after 24 h of treatment. Axio Observer with Zen Software (Zeiss). Scale bars represents 20 µm.

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software, Staining, Marker

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Impact of the compounds on cell migration. ( A , B , C ) A scratch was made into plated cells (F98) after 36 h. Cells were then treated with 0.5 µM ( A ) and 5 µM ( B , C ) of the compounds. Representative images were taken (Olympus × 71 with cell F-Software (Olympus)). ( B ), quantified and related to the time the scratch was made (0 h) ( A , C ). n = 3. * indicates significance to control ( p < 0.05).

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Migration, Software

Journal: Frontiers in Immunology

Article Title: Changes of IgG N -Glycosylation in Thyroid Autoimmunity: The Modulatory Effect of Methimazole in Graves’ Disease and the Association With the Severity of Inflammation in Hashimoto’s Thyroiditis

doi: 10.3389/fimmu.2022.841710

Figure Lengend Snippet: The changes in the content of IgG N -glycans from patients with Graves’ disease before (GD, n = 62) and after (GD/T, n = 47) stabilization of TSH level as a result of the treatment with methimazole, and the control group (CTR, n = 90). The chromatograms obtained by UPLC-MS from the separation of IgG N -glycans were manually integrated into 25 glycan peaks (GPs) and N -oligosaccharide structures were assigned based on GU values and m/z ratios using Waters UNIFI scientific information system software. N -glycan structures in Oxford notation were drawn in Sugar Bind software ( https://sugarbind.expasy.org/ ). GPs with statistically significant differences in the content of sugar structures between the study groups (GD, GD/T) and the control group (CTR) are indicated in blue. Statistical analysis was performed using the Kruskal-Wallis non-parametric test, p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). A1-2, number of antennas; B, bisecting N -acetylglucosamine; F, fucose; G, galactose, M, mannose; S, sialic acid.

Article Snippet: N -oligosaccharide structures were assigned based on the GU values and m/z ratios using Waters UNIFI scientific information system software ( , and ).

Techniques: Control, Glycoproteomics, Software

Journal: Frontiers in Immunology

Article Title: Changes of IgG N -Glycosylation in Thyroid Autoimmunity: The Modulatory Effect of Methimazole in Graves’ Disease and the Association With the Severity of Inflammation in Hashimoto’s Thyroiditis

doi: 10.3389/fimmu.2022.841710

Figure Lengend Snippet: The changes in the content of IgG N -glycans from patients with Hashimoto’s thyroiditis patients (HT2, n = 113) and the donors with the elevated IgG anti-thyroglobulin (TgAb) and/or anti-thyroperoxidase (anti-TPO) levels without symptoms of hypothyroidism (HT1, n = 68), and the control group (CTR, n = 90). The chromatograms obtained by UPLC-MS from the separation of IgG N -glycans were manually integrated into 25 glycan peaks (GPs) and N -oligosaccharide structures were assigned based on GU values and m/z ratios using Waters UNIFI scientific information system software. N -glycan structures in Oxford notation were drawn in Sugar Bind software ( https://sugarbind.expasy.org/ ). GPs with statistically significant differences in the content of sugar structures between the study groups (HT1, HT2) and the control group (CTR) are indicated in blue. Statistical analysis was performed using the Kruskal-Wallis non-parametric test, p < 0.05 (*) and p < 0.01 (**). A1-2, number of antennas; B, bisecting N -acetylglucosamine; F, fucose; G, galactose, M, mannose; S, sialic acid.

Article Snippet: N -oligosaccharide structures were assigned based on the GU values and m/z ratios using Waters UNIFI scientific information system software ( , and ).

Techniques: Control, Glycoproteomics, Software